Rubi Fructus is a well known remedy for female infertility, especially due to lack of kidney-yang or shenyangxu(???). An attempt was made to evaluate the influences of Rubi Fructus (mature Rubus crataegifolius BUNGE, mature Rubus coreanus MIQ and immature Rubus coreanum MIQ) on the morphological changes of the ovary. The experiment was performed to investigate the effects of the seeds of mature Rubus cratagifolius BUNGE (W1), the seeds of mature Rubus coreanus MIQ (W2) and the seeds of immature Rubus coreanus MIQ (W3) on the formation of follicles in rat ovary.
The results are as follows:
1. Results using three herbs of mature seed of Rubus cratagifolius BUNGE (W1), mature seed of Rubus coreanus MIQ (W2)and immature seed of Rubus coreanus MIQ (W3) clearly showed the stimulating effects on the increasing number of follicles in rat ovary. Of them, W1 increased the follicle formation by 171% compared to normal control after 4 days of treatment. In cases of W2 and W3, the formed numbers of the follicle were increased to 228% and 285%, respectively. On the other hand, the follicle formations of W1, W2, and W3 were most effective, showing 208, 258 and 266%, respectively, after 8 days of treatment. These results showed that effect of immature Rubus coreanus MIQ was similar to that of the mature Rubus coreanus MIQ without any differences.
2. The size of the follicle stimulated by W2 was about 1.0 mm, although the size of the normal control was 0.3 mm. However, those of W1 and W2 were about 0.5 mm, indicating that the treatments of the herbal extracts are also effective for the follicle size. Therefore, it was concluded that all these herbs showed the effects on average of follicles in ovary. Especially, mature Rubus coreanus MIQ showed the highest effect among them.
3. Fractions of W1-B, W2-B and W3-B, when each extract were separated by analytical HPLC(high performance liquid chromatography), showed the activities of follicle number and size of follicles in ovary.
These results suggest that the effect of Rubi Fructus is related to treatment of female infertility due to ovulation difficuty. Of them, mature Rubus coreanus MIQ is the most potent effector on the ovulation in Rats.
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